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Bulked segregant analysis was used to identify RAPD markers that display tight linkage to the V f gene in apple ( Malus sp.) that confers resistance to five races of apple scab [ Venturia inaequalis (Cke.) Wint.]. We identified several new RAPD markers linked to V f . The most tightly linked marker in the test population, S5 2500 , was cloned and sequenced. A linkage map of the V f region was developed using these markers, RAPD markers previously described by other laboratories, and the isozyme locus Pgm-1. An assay was developed for V f by multiplexing the two markers closely flanking the V f locus. This assay has a theoretical `escape' value (discarding a resistant plant) of 3% and an error rate (selection of a susceptible plant) of 0.02%.
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Hemmat et al. (1998) studied this question.
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