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An intratracheal flow rate of 20 ml/min during lung decellularization better preserved collagen and elastic fiber content compared with controls.
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A 20 ml/min intratracheal flow rate optimizes extracellular matrix preservation and lung mechanics during lung decellularization in mice.
AIM: To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol. MATERIALS & METHODS: Healthy mice were used: 15 for decellularization and five to serve as controls. Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups. RESULTS: The 20 ml/min rate better preserved collagen content in decellularized lungs. Elastic fiber content decreased at 5 and 10 ml/min, but not at 20 ml/min, compared with controls. Chondroitin, heparan and dermatan content was reduced after decellularization. CONCLUSION: An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
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Bellezzia et al. (2018) studied Healthy mice (lung decellularization) (n=20). Intratracheal flow rates vs. Ex vivo control lungs was evaluated on Extracellular matrix content and lung mechanics. An intratracheal flow rate of 20 ml/min during lung decellularization better preserved collagen and elastic fiber content compared with controls.
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