Impact of Various Desensitizing Agents on the Micro-Tensile Bond Strength of Adhesive Systems: In-Vitro Study

Objectives: To assess the influence of various desensitizing agents on the micro-tensile bond strength of various adhesive systems. Methods: Fifty extracted human permanent third molars, were sectioned at the cementoenamel junction in order to expose the mid-coronal dentinal tubules in parallel to the occlusal surface using a diamond disc and water coolant. The samples were divided into five groups and each sample was immersed in the respective desensitizing agent (Groups 1 to 5; Group 1- Distilled water, Group 2- Arginine calcium carbonate (Sensitive Pro-Relief Colgate- Palmolive, India, Group 3- Calcium sodium phosphosilicate (Repair and Protect, GlaxoSmithKline, India), Group 4- Strontium chloride hexahydrate 10% w/w (ICPA Health Products Ltd, India.), Group 5- Er,Cr: YSGG (Waterlase MD, Biolase, Irvine, CA, USA) with each group having a sample of n = 10. Each group was further divided into two subgroups (n = 5) based on the adhesive system (either a One-step self-etch adhesive (Xeno v+) (SE) or a two-step etch and rinse adhesive (Prime and Bond NT) (EandR)). Instron Micro tensile Tester was used to analyze the Micro-Tensile Bond Strength (MTBS) of the samples. Results: The bond strength of the control group (Group 1) was greater as compared to the other four groups, though statistically insignificant. There was a significant difference in the bond strength of the two subgroups (p<0.001) One-step self-etch adhesive (SE, Xeno V+) and two-step etch and rinse adhesive (EandR; Prime and Bond NT) adhesive. Conclusion: The use of desensitizing agents does not affect the micro tensile bond strength of adhesives used for the management of restoration of Non-Cervical Carious Lesions (NCCL). Microtensile bond strength with a self-etch adhesive system was significantly lower in the desensitized dentin irrespective of the agents used.