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Chlorophyll extraction (TCHL) and spectrophotometric (CM45) techniques were compared with a visual (VISINDX) method of indexing chlorosis on spinach (Spinacea oferacea L.) leaves that show various degrees of white rust (Albugo occidentalis G.W. Wils.) infection. The CM45 method gave a good fit (R 2 = 0.91) when its readings were regressed against MSINDX ratings. Both regression and cluster analyses indicated that the CM45 method was more efficient than the TCHL method in separating leaves into unique chlorosis severity classes.
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Jose Reynaldo A. Santos (1992) studied this question.
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