Development of an Active Pulsed System for Long-Term Monitoring of the Plant Photosynthetic Apparatus State Under Variable Light Conditions

We present an active pulsed system (APS) designed for the long-term monitoring of plant photosynthetic efficiency. The specific design features and technical specifications of the system are described in detail. Experimental evaluations, conducted using both a model fluorophore and live plants, demonstrated the instrument’s robust performance across a broad range of illumination, temperature, and humidity conditions. Furthermore, the results confirm the validity and reliability of the acquired data. The system’s capability to detect the onset and progression of plant stress induced by moisture deficit was successfully verified.