A bacterium YT-25 which produces enzymes lytic against Pseudomonas aeruginosa was isolated from soil and it was identified as Bacillus subtilis. A1-enzyme, A2-enzyme, B-enzyme and NLF (Native Cell-Lytic Factor) which contribute the lysis of P. aeruginosa were purified from the culture filtrate of strain YT-25. Purified A1-enzyme, A2-enzyme and B-enzyme individually lysed the vegetative cells of P. aeruginosa in the presence of NLF. NLF is a low molecular basic peptide and seemed to alter the sufrace structure of P. aeruginosa. B-enzyme hydrolyzed the peptidoglycan purified from P. aeruginosa to release the reducing groups, but A1-enzyme and A2-enzyme released neither reducing groups nor free amino groups from the peptidoglycan.
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Murao et al. (1974) studied this question.