Key result
Formalin was the least disruptive fixative for two-photon fluorescence spectroscopy of mouse skeletal muscle tissue, whereas methanol significantly influenced one of the autofluorescence peaks.
Formalin is a less disruptive fixative than methanol for preserving endogenous fluorophors in mouse skeletal muscle for two-photon fluorescence spectroscopy.
Supports formalin preference in preclinical muscle spectroscopy; hypothesis-generating before cardiac tissue translation.
We investigated the effects of handling and fixation processes on the two-photon fluorescence spectroscopy of endogenous fluorophors in mouse skeletal muscle. The skeletal muscle was handled in one of two ways: either sectioned without storage or sectioned following storage in a freezer. The two-photon fluorescence spectra measured for different storage or fixation periods show a differential among those samples that were stored in water or were fixed either in formalin or methanol. The spectroscopic results indicate that formalin was the least disruptive fixative, having only a weak effect on the two-photon fluorescence spectroscopy of muscle tissue, whereas methanol had a significant influence on one of the autofluorescence peaks. The two handling processes yielded similar spectral information, indicating no different effects between them.
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Xu et al. (2000) studied Mouse skeletal muscle. Handling and fixation processes (formalin, methanol, water) was evaluated on Two-photon fluorescence spectroscopy of endogenous fluorophors. Formalin was the least disruptive fixative for two-photon fluorescence spectroscopy of mouse skeletal muscle tissue, whereas methanol significantly influenced one of the autofluorescence peaks.
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