The influence of eluent temperature on efficiency in high-performance liquid chromatography with special reference to the role of viscous heat dissipation is investigated for water-jacket and air-bath thermostatted columns. When separations are performed on well-thermostatted large-diameter columns packed with small particles or on analytical columns operated at high flow-rates, the additional thermostatting of the eluent to a specific temperature below the column temperature turns out to have some advantages. The precooled eluent causes the development of a temperature profile in a radial direction which is opposed to and compensates the radial temperature profile in the column caused by viscous heat dissipation. For columns that are thermostatted in an air bath, a similar effect is observed, but the strong dependence of the column temperature on the eluent temperature results in a poor reproducibility if the eluent is not thermostatted itself.
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Welsch et al. (1996) studied this question.
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