The spin reveals all: Electron paramagnetic resonance spectroscopy reveals the formation of a nickel–alkyl bond in the active site of methyl-coenzyme M reductase (MCR), the key enzyme of methanogenesis, when active MCRred1 reacts with the irreversible inhibitor 3-bromopropane sulfonate to form the MCRBPS species.
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Hinderberger et al. (2006) studied this question.
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