An immunofluorescence study on the specificity of antibodies synthesized in separate cells after the administration of an immunogen with double specificity

The question whether cells can synthesize antibodies with two specificities was studied in lymph nodes of rabbits. The immunogen was poly‐D L ‐ alanylated human serum albumin which possesses distinct antigenic specificities. Tests were carried out with antibodies, conjugated to fluorescein and rhodamine, directed against the poly‐alanine and the protein moieties of the immunogen. Onwards the 20th postimmunization day, no cells with double specificity were detected.