Key result
In situ hybridization using digoxigenin-labeled non-radioactive probes successfully detected homologous and heterologous infectious bursal disease viruses in chicken bursal tissue sections.
In situ hybridization with non-radioactive digoxigenin-labeled probes successfully detects infectious bursal disease virus in chicken bursal tissue.
May aid lab detection of IBDV in poultry research; leaves open clinical translation and validation in practice.
The in situ hybridization assay was developed for the detection of infectious bursal disease virus (IBDV) infections in chickens. Bursal tissue samples were harvested 4 days following infection with the ST-C, MD, E, IN, or SAL IBDV strain. The cDNA clones STC-243, located on genome segment A, and STC-119, located on genome segment B, were used to prepare non-radioactive probes. Probes were labeled with digoxigenin and detected the homologous ST-C virus and also heterologous viruses in bursal tissue sections. No positive cells were observed in tissue sections from uninfected control chickens.
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Jackwood et al. (1992) studied Infectious bursal disease virus (IBDV) infections. In situ hybridization assay with non-radioactive probes vs. Uninfected control chickens was evaluated on Detection of homologous and heterologous IBDV in bursal tissue sections. In situ hybridization using digoxigenin-labeled non-radioactive probes successfully detected homologous and heterologous infectious bursal disease viruses in chicken bursal tissue sections.
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