What question did this study set out to answer?

Isolate pepsitensin from pepsin-digested ox plasma and determine its chemical identity and physiological properties relative to valyl-5 angiotensin I.

November 1, 1965Biochemical JournalOpen Access

Isolation and identification of a pepsitensin

View Full Paper

Ask AI

Bookmark

Authors

MFMT De Fernández APAC Paladini ADA. E. DeliusInstituto de Química y Fisicoquímica Biológicas

Discussion

Member takes

Implication

Biochemical analysis demonstrates pepsitensin from pepsin-treated ox plasma is identical to valyl-5 angiotensin I, indicating enzymatic generation of active pressor peptides.

Key Points

Isolate pepsitensin from pepsin-digested ox plasma and determine its chemical identity and physiological properties relative to valyl-5 angiotensin I.
Purified pepsitensin from ox plasma incubated with pepsin using fractional precipitation, solvent extraction, column chromatography, countercurrent distribution, and paper chromatography.
Characterized the purified peptide via specific pressor activity assays, paper electrophoresis at multiple pH values, amino acid composition analysis, and N- and C-terminal sequencing following chymotrypsin digestion.
Pepsitensin demonstrated the same specific pressor activity, amino acid composition, and paper-electrophoretic mobilities at various pH values as synthetic valyl-5 angiotensin I.
End-group analysis and enzymatic degradation confirmed the decapeptide sequence Asp-Arg-Val-Tyr-Val-His-Pro-Phe-His-Leu, establishing identical chemical structure with valyl-5 angiotensin I.

Cite This Study

Fernández et al. (1965) studied this question.

synapsesocial.com/papers/6a9e7bdcfb84d39a809bce9c https://doi.org/10.1042/bj0970540

View Full Paper

Ask AI

Bookmark

Implication

Biochemical analysis demonstrates pepsitensin from pepsin-treated ox plasma is identical to valyl-5 angiotensin I, indicating enzymatic generation of active pressor peptides.

Isolation and identification of a pepsitensin

Discussion

Member takes

Implication

Key Points

Cite This Study

Isolation and identification of a pepsitensin

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider