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January 1, 1982Applied and Environmental MicrobiologyOpen Access

Bacillus subtilis "rec assay" test with isogenic strains

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Abstract

The Bacillus subtilis "rec assay" test, widely used for the detection of DNA-damaging agents, was studied in detail, using an isogenic set of strains carrying different mutations in repair or recombination functions or both. recE4 and rec-45 mutations turned out to confer on the cells the highest sensitivity to known mutagens. The recE4 strain and its isogenic rec+ strain have been tested and validated by several rec assay procedures for preferential killing by known DNA-damaging agents. The use of purified spores of the tester strains offers advantages for standardization of the assay.

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Cite This Study

G. Mazza (1982) studied this question.

synapsesocial.com/papers/6a9e7c10dc8f3556e506858a https://doi.org/10.1128/aem.43.1.177-184.1982

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