An antitumor glucan (SSG) was isolated from the culture filtrate of Sclerotinia sclerotiorum IFO 9395 by sequential use of ethanol precipitation, ion exchange chromatography on diethyl-aminoethyl-Sephadex A-25, and precipitation with ammonium sulfate. From the results of methylation analysis and carbon-13 nuclear magnetic resonance spectroscopy, SSG was concluded to be a β-1, 3-D-glucan branched at position 6 of every two 3-substituted β-glucosyl units. SSG formed a complex with congo red in dilute alkali solution, and showed potent antitumor activity against the solid form of sarcoma 180 in ICR mice.
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Ohno et al. (1986) studied this question.