Laccase production by polyporus versicolor on different substrates

Extracellular production of laccase (E.C. 1.10.3.2) by Polyporus versicolor was studied on lignin, complex and defined media. Although the production of enzyme was more on lignin the specific activity was more on malt extract. Laccase was produced on all the media tested i.e. phenolic compounds, lignins and sugars alone, and in combination with malt extract, excepting salicylic acid. On single source media maximum yields of enzyme were obtained on polyfon followed by resorcinol, reax and lignin. As compared to phenolic compounds the enzyme production was low on sugars. Addition of malt extract enhanced the enzyme yield which was maximum in the case of lignin followed by gallic acid.