Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41

Bacillus amyloliquefaciens MIR‐41 produces three exocellular amylolytic enzymes: α‐amylase, pullulanase and α‐glucosidase. Physicochemical factors such as pH and/or temperature may affect the enzyme yield. Continuous culture at a dilution rate of 0.15 h−1 demonstrated that the maximum production of α‐amylase and α‐glucosidase occurred at 36°C and pH 6.8 and of pullulanase at 44°C and pH 5.6. The results indicated that combinations of temperature and pH might be used for the production of crude extracts of α‐amylase or pullulanase.