Key result
A recombinant porcine sapovirus strain was identified in one out of 251 faecal samples from diarrhoeic piglets in the Czech Republic, marking its first description in domestic pigs in the country.
Case Report (n=251)
Yes
This study provides the first description of a recombinant porcine sapovirus in domestic pigs in the Czech Republic.
First detection of recombinant sapovirus in Czech piglets signals need for local surveillance; leaves open its role in porcine diarrhea.
Porcine sapoviruses, members of the family Caliciviridae, have been considered as an aetiological agent of gastroenteritis in pigs. In this study, we analysed 251 faecal samples obtained from 3 to 90 day-old diarrhoeic pigs in the Czech Republic between January 2005 and June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. The presence of sapovirus (SaV) was confirmed by an RT-PCR assay with primers specific for the sapoviral RNA polymerase and capsid genes. Phylogenetic analysis based on a partial sequence of the RNA polymerase gene placed the new Czech isolate into the GVII genogroup of porcine sapoviruses; however, analysis of a portion of the capsid gene sequence classified the isolate as GIII of the genus Sapovirus. These contradictory findings indicate that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Republic
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Dufková et al. (2011) conducted a case report in Diarrhoea (n=251). Sapovirus was evaluated on Presence of sapovirus. A recombinant porcine sapovirus strain was identified in one out of 251 faecal samples from diarrhoeic piglets in the Czech Republic, marking its first description in domestic pigs in the country.
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