Using mathematical equations that describe the 0 2 mass-transfer and the enzymatic oxidation of the organic substrates of apples (Malus domestica Borkh.), we developed a kinetic model to correlate fruit respiration rate with environmental oxygen partial pressure (PO 2 . The kinetic determinations were carried out at room temperature using apples stored at 3 to 4C for 11 to 19 weeks. Results show that: 1) the calculated value of the Michaelis-Menten constant related to the enzymatic oxidation of the respiratory substrate (K m = 2.1 ± 0.5.10 -5 mol·kg -1 ) is close to that reported in the literature for cytochrome-c oxidase; 2) the located range of PO 2 levels where 0 2 becomes the limiting factor in the respiration process (near 2.6 kPa at T = 20.5 ± lC) is close to those usually used on a commercial scale for controlled atmosphere storage.
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Andrich et al. (1991) studied this question.
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