A fluorescent varient of the glmS ribozyme was used to develop a fluorescence polarization-dependent screeening and metabolite-detection assay. The metabolite-induced in-cis cleavage of the naturally occurring ribozyme could be shown to be stable and allows efficient monitoring of the ribozyme-catalyzed reaction even in bacterial extracts. The standard deviations and z′ values of the assay format indicate the high-throughput screening compatibility of the applied system for the identification of compounds that act on RNA structures.
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Mayer et al. (2006) studied this question.
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