Media Comparison for the Enumeration and Recovery of Clostridium Sporogenes P.A. 3679 Spores

The efficiency of trypticase peptone agar (TPA), yeast extract agar (YEA), and pork pea infusion agar (PPIA) for enumerating heat activated Clostridium sporogenes : P.A. 3619 spores was tested using a pour plate procedure. All three media types gave reproducible results between test runs, however TPA and YEA gave higher counts than PPIA. The three media types listed above plus pork infusion agar (PIA) and T‐Best agar (TBA) were evaluated for recovery of heat stressed P.A. 3679 spores using a similar pour plate procedure. Andersen's pork pea infusion gave the highest counts, although the variation found between tests indicates that the pour plate procedure may not be adequate for recovery of heat stressed spores.