High performance liquid chromatographic analysis of 1‐alkyl‐2‐acyl‐ and 1‐alkyl‐3‐acyl‐sn‐glycerols

A high performance liquid chromatographic (HPLC) method is described for separation and quantitation of 1‐alkyl‐3‐acyl‐ and 1‐alkyl‐2‐acyl‐sn‐glycerol, products of the detritylation reaction of 1‐alkyl‐2‐acyl‐3‐trityl‐sn‐glycerol. The alkyl glycerides were separated on a 25 cm×4.6 mm ID column packed with ∼5–6 μm silica and eluted isocratically with isooctane/isopropanol (98∶2, v/v) as mobile phase. The good separation and linear refractive index (RI) detector responses using cholesterol as an internal standard indicated the applicability of the method not only for the quantitative determination of the alkylglycerols but also for their semipreparative isolation. This HPLC method shows excellent reproducibility and accuracy and is applicable to other types of glycerides such as mono‐ and diacylglycerols.