Excretion of Fluorescein in the Urine of Women With Interstitial Cystitis

No AccessJournal of UrologyClinical Urology: Original Articles1 Sep 1997Excretion of Fluorescein in the Urine of Women With Interstitial Cystitis C.A. Tony Buffington and Bruce E. Woodworth C.A. Tony BuffingtonC.A. Tony Buffington and Bruce E. WoodworthBruce E. Woodworth View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)64316-7AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Altered bladder permeability may have a role in the pathogenesis of interstitial cystitis. Fluorescein, a fluorescent dye of molecular weight 325, has been used to assess membrane permeability. Orally ingested fluorescein normally is rapidly conjugated to glucuronate by the liver and excreted in the urine. Materials and Methods: To test its use as a marker of bladder permeability, we administered fluorescein orally to 6 patients with interstitial cystitis who satisfied National Institutes of Health, National Institute for Diabetes and Digestive and Kidney Diseases criteria and to 6 normal female control subjects. After emptying the bladder and collection of a baseline blood sample, fasted subjects ingested 20 mg. fluorescein and blood samples were collected 1, 2, 3, 4 and 24 hours later. Urine was collected during each of the first 4 hours, and then from 4 to 10, 10 to 16 and 16 to 24 hours. Urine volume was measured, and all plasma and urine samples were analyzed for fluorescein. Results: Plasma fluorescein concentrations (ng./ml.) were significantly (p <0.05) higher in interstitial cystitis patients than in control subjects at 1 and 2 hours after fluorescein ingestion. Urine fluorescein excretion (mg.) was significantly (p <0.05) lower in interstitial cystitis patients than in control subjects at 4 to 10 hours after fluorescein ingestion, and for the entire 24 hours. Conclusions: The increased fluorescein concentration in the plasma and decreased excretion in the urine of interstitial cystitis patients suggest that fluorescein may be a useful marker of altered membrane permeability. References 1 : Permeability and ultrastructure of human bladder epithelium.. Brit. J. Urol.1983; 55: 488. Google Scholar 2 : Epithelial dysfunction in nonbacterial cystitis (interstitial cystitis).. J. Urol.1991; 145: 732. Abstract, Google Scholar 3 : Bladder surface glycosaminoglycans: an epithelial permeability barrier.. J. Urol.1990; 143: 139. Abstract, Google Scholar 4 : Effect of interstitial cystitis on drug absorption from urinary bladder.. J. Pharm. Exp. Ther.1994; 271: 818. 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Google Scholar From the Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University and Riverside Methodist Hospital, Columbus, Ohio.© 1997 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 158Issue 3September 1997Page: 786-789 Advertisement Copyright & Permissions© 1997 by American Urological Association, Inc.MetricsAuthor Information C.A. Tony Buffington More articles by this author Bruce E. Woodworth More articles by this author Expand All Advertisement PDF downloadLoading ...