A technique for securing the gut contents of small, live fish consists of injecting water from a hypodermic syringe through the alimentary tract by means of a short tube that is inserted into the intestine at the anus. The gut contents are flushed out through the mouth. Experiments on killifishes, Fundulus spp., indicate a nearly 100% efficiency in emptying the gut and no detrimental effects from multiple injections. The technique does not work equally well for all species of small fishes.
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Baker et al. (1976) studied this question.
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