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A fluorescence microscope for the detection of spatially resolved single molecules at low temperature has been developed. The main part consists of a microscope objective which is inserted in superfluid helium. Two versions of the optical system are described. Both setups have a high numerical aperture and thus high collection efficiency. A video camera with an image intensifier is used to detect the fluorescence images. Detection of single molecule fluorescence has been achieved. It was even possible to visualize individual fluorescing molecules on a video monitor in real time. Spectroscopic applications of this parallel detection scheme are discussed.
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Jasny et al. (1996) studied this question.
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