Receptor Substance for Pyocin R

Pyocin Rreceptor substance was prepared from Pstudomonas atruginosa Pll (pyocin R sensitive strain) by trichloroacetic acid extraction, nucleate digestion and pronaae digestion. Most of the receptor substance was precipitated by ultracentrifugation. 2. This substance was mainly composed of lipopolysaccharide and contained glucose, rhamnose, glucosaminc, galactosamine, heptose and a sugar giving a positive reaction with thiobarbituric acid in the carbohydrate moiety. It contained a small amount of amino acids. 3. The receptor activity decreased on chloroform-methanol extraction of the lipid, on alkali treatment or on periodate oxidation. 4. The TCA extract from the pyocin R resistant mutant (Pll Rr) had no receptor activity and no corresponding precipitates could be obtained from the extract by ultra-centrifugation. 5. The TCA extraction method was more effective than the phenol method for preparation of lipopolysaccharide with receptor activity.