A model system consisting of oil droplets in a glycerol-water matrix was freeze-cleaved and subjected to prolonged treatment with diethyl-ether at dry ice temperature before etching and shadowing. Dissolution of droplets became visible after some hours. Complete fat extraction resulted in a uniform appearance of the replica with holes replacing the droplets. Possibilities of this new method for the demonstration of membrane lipids in vitrified cells are discussed.
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Richter et al. (1971) studied this question.