Histological studies on embryos produced from cell cultures of Bromus inermis

A histological study has been made of the embryos produced in a brome cell suspension culture. In 2,4-dichlorophenoxyacetic acid-free defined medium, several types of cell aggregates were produced. Some of the aggregates were proembryo-like structures. A frequent intermediate in embryogenesis was a "tadpole"-like structure. Shoot and root primordia were produced within the proembryonic structures. Plant development proceeded by the formation of a shoot–root axis, then roots developed and later the coleoptile and leaves were produced.