Key result
Three novel monoclonal antibodies against HCV core protein were established and successfully used to detect HCV core antigen in cell lines and chronically infected chimpanzee liver tissue.
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Three novel monoclonal antibodies against HCV core protein were established and characterized, providing important reagents for HCV biology study and immunodiagnostic assay development.
Three novel monoclonal antibodies (MAbs) were established against a recombinant hepatitis C virus (HCV) core protein derived from cloned genotype 1b HCV cDNA. MAbs C7-50 and C8-59 recognize a conserved linear epitope represented by amino acid residues 21 to 40 of the nucleocapsid protein. MAb C8-48 is directed against a strain-specific conformational epitope located within the first 82 amino acids. A sensitive two-site MAb-based immunoradiometric assay was established using antibodies directed against distinct epitopes on the nucleocapsid protein. Processed 21 kDa core protein was detected by immunoblotting in human hepatocellular carcinoma cell lines and primary adult rat hepatocytes transfected with a cytomegalovirus promoter-driven expression construct. Immunofluorescence microscopy studies revealed a granular and vesicular cytoplasmic staining pattern. MAb C7-50 was used successfully to detect HCV core antigen in chronically infected chimpanzee liver tissue. These MAbs represent important reagents for the study of HCV biology and for the development of immunodiagnostic assays.
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Moradpour et al. (1996) studied Hepatitis C virus (HCV). Monoclonal antibodies (C7-50, C8-59, C8-48) against HCV core protein was evaluated on Antibody characterization and detection of HCV core antigen. Three novel monoclonal antibodies against HCV core protein were established and successfully used to detect HCV core antigen in cell lines and chronically infected chimpanzee liver tissue.
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