Structural Analysis and Antimicrobial Mechanism of a Protein GBSPI-A from Ginkgo Biloba Seed

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Ginkgo biloba seed has antimicrobial activity. In this study, ginkgo biloba seed protein was prepared, identified, and named GBSPI-A, finding its construction was similar to 11-S globulin. Then, the influence of GBSPI-A on the cell membrane and physiological metabolism of K. pneumoniae and S. aureus were investigated. The results showed that GBSPI-A (20 mg/mL) destroyed the cell membrane, causing leakage of intracellular material and inhibited bacterial growth with an inhibition rate of approximately 80%. In addition, the GBSPI-A (10 mg/mL) caused the decreasing activity of ATPase and respiratory rate, and the respiratory depression rate was 7.24%. Furthermore, the decreasing ATP synthesis and intracellular β-galactosidase activity led to an insufficient supply of physiological metabolic energy. Therefore, the results showed that GBSPI-A could be used as a natural bacteriostatic agent to replace related drugs and also provide a new insight into the application of GBSPI-A in food safety.