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The histochemical method to demonstrate L-leucyl-beta-naphtylamidase (LNAase) activity has been applied to biopsies of normal human ductus deferens, deferential ampulla, seminal vesicle, prostate, bulbourethral (Cowper's) gland, and cavernous urethra. An intensely positive reaction was observed in the secreting epithelia of prostate, Cowper's, and urethral (Littré's) glands. Not only the prostate but also the Cowper's and Littré's glands secrete some LNAase into the semen. A possible role of the seminal LNAase is discussed.
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Cossu et al. (1988) studied this question.
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