An apparatus is described in which spectra of microscopic objects can be continuously scanned. An adjustable linear spiral slit rotated at 2 rps provides an oscillographic presentation of either emission or absorption spectra. The apparatus was developed primarily for the study of fluorescent structure in connection with the photochemistry of living cells. An alternate modification provides the simultaneous recording of the time course of spectral changes at several selected wavelengths. Performance data and examples of biological application are presented.
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Rodney A. Olson (1960) studied this question.
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