Electrocatalytic reduction of hemoglobin at a chemically modified electrode containing riboflavin

Chemically modified electrode (CME) exhibting electrocatalytic response toward hemoglobin (Hb) is constructed by constructed by absorbing the riboflavin mediator onto spectroscopic graphite surface. The electron transfer between the riboflavin functionality and the graphie is fast. The surface coverage is at most 9.6 × 10−10 mol cm−10. The modified electrode permits the electroreduction of hemoglobin to take place at a low potential corresponding to the reduction potential of the mediator molecule. For neutral and slightly acidic solutions, this corresponds to very modest negative potentials (vs.Ag/AgCi). Characterization of the performance of the CME is carried out. After 30 min of continuous potential scanning of pH6.8, 60% of the original coverage remains for the CME. The linear range of the calibration plot is 30–480 μmol/L. The effect of the solution pH on the electrocatalytic activity of the CME for hemoglobin reduction is investigated. Due to its high sensitivity and durability, this CME coulde be used as an analytical sensor for detecting hemoglobin in real samples.