Alternate Isotope‐Coded Derivatization Assay: An Isotope Dilution Method Applied to the Quantification of Zearalenone in Maize Flour

Low-cost isotope dilution made easy: An alternate reaction of an analyte in a matrix and in a standard solution with isotope-labeled or unlabeled derivatizing reagents allows quantification of zearalenone in maize flour without the need for a calibration curve. This is possible by direct calculation of the relative response factor for the analyte/internal standard ratio of the sample (see picture).