Key result
The Rep protein encoded by Wheat dwarf virus acts as an RNA silencing suppressor by binding 21 nt and 24 nt siRNA duplexes and single-stranded siRNA.
The Rep protein of Wheat dwarf virus is identified as the first RNA silencing suppressor encoded by a mastrevirus.
Identifies first mastrevirus RNA silencing suppressor; extends geminivirus evasion models but leaves open in planta validation.
Wheat dwarf virus (WDV) is a DNA virus belonging to the genus Mastrevirus of the family Geminiviridae. In this study, we report that the Rep protein encoded by WDV is a RNA silencing supressor as determined by co-infiltration assays using transgenic Nicotiana benthamiana line 16c carrying the GFP reporter gene. The Rep protein was shown to inhibit both local and systemic RNA silencing of the GFP gene as well as the spread of systemic GFP RNA silencing signals. Gel mobility shift assays showed that the Rep protein binds 21 nt and 24 nt small interfering RNA (siRNA) duplexes and single-stranded (ss)-siRNA. To our knowledge, this is the first identification of an RNA silencing suppressor encoded by mastreviruses. Furthermore, deletion mutagenesis indicates that both the N- and C-terminal regions of the Rep protein are not critical for silencing suppression and self-interaction, but the N terminus of Rep is necessary for its pathogenicity.
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Wang et al. (2014) studied Wheat dwarf virus infection. Rep protein encoded by WDV was evaluated on RNA silencing suppression and siRNA binding. The Rep protein encoded by Wheat dwarf virus acts as an RNA silencing suppressor by binding 21 nt and 24 nt siRNA duplexes and single-stranded siRNA.
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