Lysophosphatidylcholine trigger myocardial injury in diabetic cardiomyopathy via the TLR4/ZNF480/AP-1/NF-kB pathway

Figures

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  Fig. 1. Gut microbiome in T2DM incident and the relationship between gut flora and clinical baseline indicators. (A) Significant differences in gut microbes between T2DM and N groups. *q < 0.05,**q < 0.01 as determined by MetaStat analysis. q < 0.05 was considered significant. (B) Functional prediction of gut microbiota with significant differences between T2DM and N groups. *p < 0.05,**p < 0.01 as determined by Taxfun algorithm. p < 0.05 was considered significant. (C) Association analysis between gut microbiota and baseline clinical characteristics of the subjects. *p < 0.05,**p < 0.01 as determined by spearman analysis. p < 0.05 was considered significant.

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  Fig. 2. Association analysis between LPC, gut microbiota, and baseline clinical characteristics of the subjects.(A) The relationship between LPC and baseline clinical characteristics of the subjects.(B) The relationship between LPC and gut microbiota. *p < 0.05,**p < 0.01 as determined by spearman analysis. p < 0.05 was considered significant.

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  Fig. 3. Association analysis of LPC and mRNAs, and RNA-DNA conjoint. (A) Association analysis between ZNF480, NFKB2, CD36, and LPC. (B) Log2FoldChange of ZNF480, NFKB2, and CD36. *p < 0.05,**p < 0.01 as determined by spearman analysis. p < 0.05 was considered significant. Purple represents down-regulation, and red represents up-regulation. (C) The GO pathway of differentially expressed genes shared at the DNA and RNA levels. Expression of (D) ZNF480, (E) NFKB2, (F)TLR4, (G) CD36, (H)c-Jun, (I) c-Fos in peripheral blood of T2DM patients. *p < 0.05, **p < 0.01 as determined by two-tailed Student's t-test, and Kruskal-Wallis H. p < 0.05 was considered significant.

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  Fig. 4. Changes of blood indexes and H&E staining of liver, pancreas, and heart tissues in N and M groups.The levels of serum (A) FPG, (B) INS of T2DM model mice. *p < 0.05,**p < 0.01 as determined by two-tailed student's t-test. p < 0.05 was considered significant. (C)The H&E staining of the liver, (D) pancreas, and (E) heart tissues. The magnification of the liver tissues is × 20. The magnification of both the pancreas and heart tissues is × 40.

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  Fig. 5. Gene expression of pancreatic and heart tissues of mice with T2DM. (A) and (B) TLR4 expression of mouse pancreatic tissues between N and M group (magnification was × 20). (C–K) TLR4, and NFKB2 expression of mouse cardiac tissue between N and M group (magnification of images C and E was × 20). (L) ZNF480, (M) TLR4, (N) c-Jun, (O) c-Fos, (P) NFKB2, and (Q) CD36 expression of mouse cardiac tissue between N and M group. *p < 0.05,**p < 0.01 as determined by two-tailed student's t-test. p < 0.05 was considered significant.