Biochemical analysis reveals shifting protein diversity and chemical composition of milk-fat globule membranes in Murrah buffaloes across lactation, highlighting progressive molecular maturation.
Chemical analyses, polyacrylamide-gel electrophoresis and isoelectric focusing of milk-fat globule membrane proteins (FGMP) obtained from the milk of 2 Murrah buffaloes were done to determine if any change in composition occurred during lactation. Changes in the levels of sialic acid, hexose, hexosamine, N and P were found in the FGMP obtained at different stages of lactation. On the day of parturition, 8 major proteins in FGMP were determined by sodium dodecyl sulphate polyacrylamide-gel electrophoresis whereas 6 major proteins were obtained in FGMP of middle and late lactation milks. Isoelectric focusing of FGMP showed 8-9, 9-13 and 13-16 proteins from colostrum, middle and late lactation milks, respectively and the isoelectric pH of the proteins varied from 5-25 to 7-80, 5-85 to 8-30 and 5-75 to 8-61 respectively.
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Singh et al. (1976) studied this question.
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