The objective of this study is to investigate the optimal parameter levels for removal of nitrates from wastewater using Pseudomonas stutzeri in a fluidized bed bioreactor by Taguchi method and validating the predicted values. In this study the influence of airflow rate, temperature, carbon source, PP beads (Poly Propylene) and pH at different levels was investigated was made using Taguchi's L16(4) 5 orthogonal array technique. The nitrate removal efficiency after optimization is more than 96% with airflow rate at level 2 (A2), temperature at level 3(B3), carbon source at level 4(C4), PP beads at level 2(D2) and pH at level 2(E2). The analysis of variance (ANOVA) test is used to determine the percent contribution of each parameter and found that airflow rate contribution is the highest and temperature provides the least contribution. Keywords - Wastewater, Biological denitrification, Fluidized bed bioreactor, Pseudomonas stutzeri, Taguchi method,
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S et al. (2011) studied this question.