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A rapid procedure was developed for staining fetal rat specimens. Full‐term rat fetuses were fixed in 99% ethanol and only thoracoabdominal organs were removed. Without skinning and removing adipose tissue and eye balls, the specimens were placed in 85% ethanol containing 1.5% KOH and 0.0015% alizarin red S for 5 days. The specimens were macerated in 1% aqueous KOH and cleared in 50% glycerin solution. The skeleton was visible through surrounding tissue containing adipose tissue which became transparent.
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Kawamura et al. (1990) studied this question.
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