Abstract 1. A sensitive, reproducible method for serum iron determination has been developed employing extraction of serum acid hydrolysate with isoamyl alcohol and development of a highly colored ferrous complex of 4,7-diphenyl-1, 1O-phenanthroline for quantitative photometric measurement of iron. 2. The method gives good recoveries and also good agreement with the dry-ashing procedure. 3. A normal range of 120-200 µg./100 ml. (average 160) for the female and 125-238 µg./100 ml. (average 182) for the male for serum iron was obtained by the method.
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Kingsley et al. (1956) studied this question.