In order to make the method of spin trapping technique with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) more powerful in biological investigation, using electrochemical reaction, we have developed the method to observe an ESR spectrum composed of only a spin adduct of hydrogen atoms (DMPO-H). It has been elucidated that DMPO-H is produced through the hydrogen atom formation, not through the reduction of DMPO.
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Endo et al. (2001) studied this question.
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