Topical Application of Epidermal Growth Factor (EGF) to Changes in Tympanic Membrane Perforation Diameter

Background and Objective: Some researches have already been conducted about the effect of topical application of Epidermal Growth Factor (EGF) in the use of amniotic graft to changes in tympanic membrane perforation diameter.The aim of this research was to know the effects of topical Epidermal Growth Factor (EGF) application in graft amnion usage on perforation membraneʼs diameter which compared to the control.Materials and Methods: The research method used was experimental with randomized pre-test and post-test control group design.Patients with chronic tympanic membrane perforation without infection were injured at the perforated edge using a sickle knife which was then smeared with a topical epidermal growth factor then perforation closes with the amniotic membrane.There were 20 samples consisting of 10 samples with amnion membrane without topical EGF and 10 samples with amnion membrane plus topical EGF.Results: The results obtained in the EGF group had significantly reduced perforated diameter (p<0.05) by 21.1% during the 2nd week; whereas in the group without EGF has not been reduced at all.This means that there was a faster decrease in perforated diameter in the topical group treated with EGF than in the top group without the topical EGF, since the 2nd week and significantly different in the 5th week.Conclusion: Epidermal growth factor plays a role in shrinking the perforation diameter of the tympanic membrane on the use of amniotic membranes as graft.