Finesse and sensitivity gain in cavity-enhanced absorption spectroscopy of biomolecules in solution

We describe a 'wet mirror' apparatus for cw cavity-enhanced absorption measurements with Bacteriochlorophyll a (BChla) in solution and show that it achieves the full sensitivity gain ( approximately 2.3 x 10(4)) afforded by the finesse (3.4 x 10(4)) and loss distribution of our optical resonator. This result provides an important proof-of-principle demonstration for solution-phase cavity-enhanced spectroscopy; straightforward extrapolation to a system with state-of-the-art low-loss mirrors and shot-noise-limited performance indicates that single molecule sensitivity in liquids is within reach of current technology. With the probe laser locked to the cavity resonance, our instrument achieves a sensitivity approximately 3.4 x 10(-8)/ radicalHz (for a sample of length 1.75 mm) with 100 kHz bandwidth and can reliably detect sub-nM concentrations of BChla with 1 ms integration time.