Bone marrow aspirates from 30 cases of acute lymphocytic leukemia and 21 cases of acute non-lymphocytic leukemia were stained with oil red O and for β-glucuronidase. Globular oil red O positivity was seen more frequently in cases of acute lymphocytic leukemia, and granular oil red O positivity was observed in a greater percentage of cases of acute nonlymphocytic leukemia. Diffuse activity or blocks of β-glucuronidase positivity were found with equal frequencies in cases of acute lymphocytic leukemia and acute non-lymphocytic leukemia. Both stains are considered to be of little value in the classification of acute leukemia.
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Shaw et al. (1974) studied this question.