Continuous enzyme processing: The isolation of prolyl‐tRNA synthetase from Phaseolus Aureus

A pilot‐plant process has been developed for the continuous extraction and partial purification of prolyl‐tRNA synthetase from mung bean. The bean slurry was wet ground in a hammer mill, clarified by two‐stage centrifugation, and the protein in the effluent fractionated by precipitation at pH values of 5.2 and 4.2. The throughput was 13 kg dry bean/hr. The improved extraction process and reduced processing time resulted in an enzyme product with a specific activity 16 × that previously obtained in the batch process. The yield was also 50–60 times higher.