Sclerotium rolfsii, grown in a shaken submerged culture, developed a potential for the formation of sclerotia. This potential was inhibited by the presence of glucose in the culture medium. The cultures were capable of producing sclerotia, after transfer to stationary incubation, when glucose was exhausted and the biomass reached its maximal level. The level of adenosine 3′,5′-cyclic monophosphate (cAMP) sharply increased upon glucose depletion and decreased upon glucose addition. Sclerotium rolfsii was grown in a chemostat with glucose of limiting concentration at a dilution rate of 0.05/h. Samples from the chemostat possessed the potential to produce initials when poured into empty Petri plates. Addition of glucose to these plates changed the fungal growth pattern from further differentiation to vegetative growth. Glucose supplementation to clearly differentiated initials did not inhibit further development. It was observed that during morphogenetic events endogenous substrates, e.g., cell wall components, were utilized.
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Hadar et al. (1983) studied this question.