Collecting single molecules with conventional optical tweezers

The size of particles that can be trapped in optical tweezers ranges from tens of nanometers to tens of micrometers. This size regime also includes large single molecules. Here we present experiments demonstrating that optical tweezers can be used to collect polyethylene oxide molecules suspended in water. The molecules that accumulate in the focal volume do not aggregate and therefore represent a region of increased molecule concentration, which can be controlled by the trapping potential. We also present a model that relates the change in concentration to the trapping potential. Since many protein molecules have molecular weights for which this method is applicable the effect may be useful in assisting nucleation of protein crystals.