Starch granule‐associated proteins and protein aggregates not associated with starch granules were visualized by staining with acid fuchsin. Based on fluorescence intensity, it was observed that non‐granule‐associated proteins could be removed from starch granule preparations by treatment with a modification of the Osborne‐Mendel fractionation method without affecting the apparent level of granule‐associated proteins. Moreover, fluorescence microscopy with direct spectrophotometric measurement of the proteins confirmed the presence of major subpopulations of granules differing in size and acid fuchsin‐induced fluorescence. Treatment of stained granules with 1.0% SDS dramatically reduced fluorescence intensity, indicating the removal of granule‐associated proteins.
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Wasiluk et al. (1994) studied this question.
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