Isolated plastids from crude extracts of sunflower (Helianthus annuus L. cv. Sungold) leaves released a factor on extraction with Triton X‐100 that inactivated Rhodotorula glutinis L‐phenylaine ammonia‐lyase (PAL; EC 4.3.1.5) in vitro. This PAL‐inactivating factor (PAL‐IF) was found to be proteinaceous in nature when tested with pronase (EC 1.11.1.6), peroxidase (EC 1.11.1.7) and nitrate reductase and the protection of PAL from PAL‐IF by ligands indicated its specificity towards PAL. The inactivated PAL inhibitors reported earlier. It is suggested that inactivation may play an important role in in vivo regulation of L‐phenylalanine ammonia‐lyase activity and phenolic biosynthesis.
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Gupta et al. (1984) studied this question.
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