Coagulation factor levels in solvent/detergent‐treated plasma

Coagulation factor levels in solvent/detergenttreated plasmaRecently, an extensive committee report on the current status of solvent/detergent (S/D)-treated frozen plasma was published by Klein et al. 1 in TRANSFUSION.Although S/Dtreated plasma is now widely used, little is published about the amount and the stability of the individual coagulation factors in S/D-treated plasma. 2,3According to the manufacturers, S/D treatment does not significantly alter the concentration of coagulation factors, and a concentration of the individual coagulation factors of at least >0.50 U per mL (50% of normal) is guaranteed.Although this level is above the hemostatic value of most coagulation factors, it may be insufficient for correction of severe deficiencies of coagulation factors.We studied the levels of several coagulation factors in seven batches of S/D-treated plasma (ESDEP, CLB, Amsterdam, the Netherlands).This S/D-treated plasma is prepared from pooled fresh-frozen plasma (FFP) from healthy unpaid Dutch blood donors.The technology used to prepare S/D-treated plasma was developed by Octopharma AG (Glarus, Switzerland). 4S/D-treated plasma was thawed in a waterbath at 37 o C for 20 minutes according to the manufacturer's instructions.Plasma samples were tested in duplicate immediately after thawing and after storage at room temperature for 2, 4, and 8 hours.Antithrombin, plasminogen, and antiplasmin were measured wtih chromogenic assays.Other coagulation factors, including protein S and protein C activity; factors II, V, VII, VIII, IX, X, XI, and XII; and fibrinogen, were determined according to standard clotting assay procedures.Protein C, protein S, and von Willebrand factor (vWF) antigen levels, as well as binding of vWF to collagen were determined by enzyme-linked immunosorbent assays.For our laboratory assays, reference pooled plasma was obtained from 40 healthy volunteers.Coagulation factors were not determined in FFP before S/D treatment.The results of the concentrations and activities of coagulation factors in S/D-treated plasma are shown in Table