Peanut oil was separated into the various lipid classes by column chromatography. The polar lipid fraction which contained phospho‐lipids was separated into individual major components by 2‐dimen‐sional thin layer chromatography. Conversion factors for calculating the concentration of total phospholipid in peanut oil from percent elemental phosphorus were determined by estimation of molecular weights for the respective components. A conversion factor of 23.6, 24.8, 26.6, 22.2, and 24.4 was found for phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidic acid, and total phospholipid, respectively. These factors also were used to convert µg of phosphorus into µg of phospholipid.
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Singleton et al. (1981) studied this question.
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